The corrected absorbance is within 5% of the. The x and y calibrations are the same 60 µm per pixel 2. M3-M5e_manual. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. -- JULY 19, 2016 Cables. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. Moving a SpectraMax Multi-Mode Microplate Reader. Rating: 5. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. Microplate Reader, For Lab. Specifications Figure A-3 Top view of a SpectraMax reader ® Common Fluorescence and Luminescence Wavelengths Values in the following tables are based on the literature. 21 MB. 1. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Our absorbance plate readers feature our PathCheck Sensor. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The mirror set. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. com. The following document is. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seSpectraMax L: 36. D 1 1. spr * SpectraMax i3(x) SpectraTest ABS2 v1. The optical systemsThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 6 M HCl for 24 h. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. Using. 2 x 10-14. Molecular Devices SpectraMax M5e User Manual (20 pages) Multi-Mode Microplate Readers. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seAn example of an upgradeable multi-mode plate reader is the SpectraMax M5e, a five-mode reader that is part of the same line as the first microplate reader used in space, thanks to its durability. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. For more information please visit us at: SpectraMax®i3Multi-ModeMicroplateReader* SpectraMax®i3xMulti-ModeMicroplateReader*. The chamber. 9000-0756. It was also read on the monochromator-based SpectraMax® M5e Multi-Mode Microplate Reader using optimized wavelengths. 1. book Page 21 Wednesday, July 7, 2010 2:09 PM. combines Molecular Devices’ SpectraMax® M5e Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. 多功能酶标仪 FlexStation3 快速动力学操. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 1. 303 unable to cal dark current. book Page 1 Wednesday, July 7, 2010 2:09 PM The Molecular Devices SpectraMax M5e Microplate Reader enables five modes of detection. Once the M5e Reader is operational, space-based researchers will be able to conduct analysis on-orbit. The SpectraMax® M5e Multi-Mode Microplate Reader from Molecular Devices delivers single-mode reader performance in one multi-mode reader package. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader; SpectraMax® M3 Multi-Mode Microplate Reader; SpectraMax® M2 and M2e Multi-Mode Microplate Readers; SpectraMax® 340PC 384 Absorbance Microplate ReaderMolecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. 3. Recommended Optics Wavelength (nm) Wavelength selection Excitation 332/9 Monochromator Emission 1 488/15. 92 0. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Explore more about Optimized settings for Transcreener TR-FRET assay on SpectraMax iD5 and SpectraMax i3x readers. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Five preferred detection modes. With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. Cellular Imaging Systems: Molecular Devices offers various imaging solutions for your research needs. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument. 312 gain calibration failed. The optical systemsProducts : SpectraMax M5/M5e reader Can BRET be read on the SpectraMax M5? BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. SoftMax Pro 5 GxP Software provides all the data collection and analysis tools of the standard edition, along withMolecular Devices. 40266 Email: drugdiscoverytech@lifetech. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. Description 1. View the Molecular Devices website. 1. Molecular Devices SpectraMax 340PC Microplate Spectrophotometer $ 3,500. 97 cAMP Detection AssayMolecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. 01 1 100 10000 1000 2000 3000 SpectraMax Paradigm SpectraMax M5e [cAMP] nM delta F % EC 50 Values Paradigm = 18nM M5e = 19nM Variation of Integration Time-12 -10 -8 -6 -4 5000 10000 15000 20000 25000 50 s delay 400 50 s. Add to cart. Choose from a three- (M3), four- (M4), orHome; Lighting; Work lights; User manual. 3. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. #M5e) Methods Microplate reader compatibility test. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Detection cartridge modularity allows for easy addition of new read modes within minutes without. After the firefly luciferase was measured, Dual-Glo Stop & Glo substrate solution (25 µL/well) was added offline to each well to quench the firefly luciferase reaction and provide substrate for the Renilla luciferase reaction. We would like to show you a description here but the site won’t allow us. 1. 305 reference level saturation. Molecular Devices SpectraMax M5e Multimode Microplate Reader. Description 1. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader. Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. Commenting on the launch, Christopher Silva, Director of Life Sciences Marketing at Molecular Devices, stated, "The HTRF(R) assay platform has given researchers an alternative set of kinase and GPCR assays, in. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeMulti-mode Microplate Readers. In the M2, M5 and Plus. Both instruments demonstrated good performance for the assay. The actual pathlength, d, of a solvent is found from the following equation: d cm. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The user manual is an indispensable documentation for of any product. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. 0. SpectraMax Mini: 17. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Multi-Mode Microplate Readers. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). On SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Results summary for cAMP HiRange standard curve Figure 3. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Introduction. NanoRacks LLC officials have chosen Molecular Devices’ SpectraMax M5e Multi-Mode Microplate Reader for use in NASA's International Space Station. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 8 x 10-15. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. Path Check Pathlength Measurement Multimode Reader Comparison Chart. The CellTiter-Glo® 2. . 5, Z’ = 0. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. SpectraMax L: 36. Table of Contents. 5 mM IPTG using a SpectraMax M5E. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader w/ One Year Warranty (Svc Contract Available) ATG for AKTA Explorer, Purifier and FPLC PMs, Repairs and Service Contracts. Supplier: Molecular Devices. Z1. Choose cables that meet the requirement described in this appendix. Each tube of fluorophore also contained ROX. With the cAMP dynamic 2 kit, cAMP levels are detected using a Eu. SpectraMax® M5e. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. 90-0083 series): Excitation 530 nm,. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. Bioz Stars score: 86/100, based on 1 PubMed citations. Upgradeable modules are also available including western blot, cell imaging, and fast. The optical systemsApplication note demonstrating that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Read mode. e. HTRF is compatible with a large range of available microplate readers. Description 1. 5 mP standard deviation at 1 nM. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. No special upgrade is required for HTRF ® readout, as it is a monochromator-based instrument. Simultaneous dual emission detection enables read times of only 2:17 for an entire 384-well plate. KeySpan adapter for SoftMax Pro and SpectraMax readers. GFP fluorescence was read on days 1, 4 and 7, using a SpectraMax M5e Microplate Reader (Molecular Devices). The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Bottom-reading is usually recommended for cell-based assay with adherent cells. The KeySpan USA-19HS is often required with new Windows 7. October 2023. SpectraMax M5e: 118. SpectraMax Mini: 17. The SpectraMax M5e possesses five preferred modes for multiple detection benefits in one platform: UV-Visible Absorbance (Abs), Fluorescence Intensity (FI), Time-Resolved Fluorescence (TRF), Fluorescence. 40266 Email: drugdiscoverytech@lifetech. The plate reader. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical-based and cell-Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader Chosen to be the First Microplate Reader in Space by NanoRacks, LLC for NASA’s International Space Station Sunnyvale, CA – May 17th, 2011 – Today, Molecular Devices, a leading provider of instruments, software, and consumables for drug discovery and life sciences research,Configure your microplate reader. Multimode Readers Comparison Chart. 2222 Fifth Street. 2. SpectraMax L: 36. Molecular Devices FilterMax F5 Multi-Mode Microplate Reader $7,900. In this. Data collected by Analyst® and FLIPR® Systems can also be imported and analyzed in the software. 6 x 10-15. Recertification is recommended every two years. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. Dual monochromators allow users to target. No sabemos si este producto volverá a estar disponible, ni cuándo. The Molecular Devices Gemini XS Fluorescent Microplate Reader lets you experiment with a variety of fluorophores and can even find the best excitation and emission wavelengths for new assays. SpectraMax Mini: 17. SpectraMax Paradigm. Molecular Devices. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 3. Introduction Inflammation, the body’s reaction to infection, irritation, or other injury, is accompanied by increased endothelial chemokine. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. 5. Paradigm: W = 21. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Upgradeable modules are also available including western blot, cell imaging, and fast. 8. G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax i3x, SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 Linearity G03 through G06forSpectraMaxiD3,SpectraMaxiD5,SpectraMax. For more sample throughput, the system canSetup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. reader (FI-FLRH) SpectraMax Paradigm. With optimized reagents, option to operate the readers. Download PDF. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. spr SpectraMax iD3 SpectraTest ABS2 v1. SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. Configure your microplate reader. Upgrades to the original platform include temperature control and the ability to configure every aspect of the researcher’s. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 1. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. book Page 55 Wednesday, July 7, 2010 2:09 PM. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. We would like to show you a description here but the site won’t allow us. SpectraMax® L (software version 5. ZERO BIAS - scores, article reviews, protocol conditions and moreThe NanoRacks Plate Reader-2 is a reconfigured SpectraMax M5e, one of Molecular Devices’ most reliable, durable, and feature-rich microplate readers, and has been configured to operate comfortably in the microgravity environment of the space station. Note: In this user guide, SpectraMax ® refers to several SpectraMax . 5. The donor and acceptor can be used to label a variety of biomolecules, for applications. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator the assay on the SpectraMax Paradigm and M5e readers was evaluated by titration curves shown below. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. Specifications Figure A-3 Top view of a SpectraMax reader ® Common Fluorescence and Luminescence Wavelengths Values in the following tables are based on the literature. SpectraMax M5e Multi-Mode Microplate Reader. For a complete list of the instruments supported by this release of the SoftMax Pro Software, see Supported Instruments on page 111. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. 3. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideGeneration of NADPH was monitored by fluorescence (Ex340/Em460 nm) every minute for 15 minutes on a SpectraMax M5e plate reader (Molecular Devices at 25°C). Introduction. 8. 2 x 10-14. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellDNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). 00We would like to show you a description here but the site won’t allow us. book Page 1 Wednesday, July 7, 2010 2:09 PM. SpectraMax i3 reader SpectraMax Paradigm reader SpectraMax M5e reader cAMP EC 50 (nM) 19. 316 grating motor driver faulty. The mirror set. HTRF cAMP calibration curve HTRF cAMP calibration curve measured on the SpectraMax i3 (red circles), SpectraMax. 315 can't find zero order. SpectraMax® iD5. Relative fluorescence units (RFU) were converted to units of NADPH concentration (μmol/L) using a standard curve of NADPH. SpectraMax Mini: 17. SPECTRAMAX XS XENON REPLACEMENT BULB FOR MOLECULAR DEVICES SPECTRAMAX XPS XENON, SPECTRAMAX XS. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. 90 Read time (384 wells) 5:03 2:17 14:00 Table 3. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. About Conquer. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. 标准功. Wide range of applications. The schematic of the UED setup is shown as Fig. 6 x 10-15. High sensitivity and. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). System setup and calibration are controlled. Molecular Devices. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. Table of Contents. com 10. 5. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. The self-funded NanoRacksSpectraMax® Paradigm® Multi-Mode Detection Platform. The Spectr. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. SpectraMax M5e: 118. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. Once the M5e Reader is operational, space-based researchers will be able to conduct analysis on-orbit. Path Check Pathlength Measurement We would like to show you a description here but the site won’t allow us. M5e: W = 15. Italic and boldface type are used for emphasis. This document is provided to customers who have purchased Molecular Devices, Inc. 3. 0 nm increments Bandwidth (EX, EM) 9 nm, 15 nm Optimized Precision ≤ 3. UT-6550 Microplate Reader is a reliable and robust instrument for a wide. Products : SoftMax Pro GxP Software, SoftMax Pro Software, SpectraMax M5/M5e reader. SpectraMax M5e: 118. The following document is intended. -- JULY 19, 2016Cables. Bioz Stars score: 86/100, based on 1 PubMed citations. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. The assay was set up in a low-volume 384- well black plate using the pipetting. The Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument se在2011年5月，凭借其耐久性，Molecular Devices SpectraMax M5e成为了第一台进入太空的微孔板读板机。NanoRacks将它加入国际空间站中的商业硬件实验平台，首次为科学家提供了在微重力环境下进行微孔板检测的机会。Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Molecular Devices Spectramax Plus Microplate UV/VIS Reader. 8. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. Description 1. The chamber contains springs that position the cuvette in the proper alignment for a read. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. softmax pro software, spectramax paradigm, spectramax m5e/m5, walk-away automation, lab automation, fda 21 cfr oart 11, glp, gmp, stakmax Created Date: 1/11/2022 12:34:59 PM. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. A suspicious death, an upscale spiritual retreat, and a quartet of suspects with a motive for murder. 0. Gemini EM. Today, Molecular Devices, a leading provider of instruments, software, and consumables for drug discovery and life sciences research, announced that NanoRacks, LLC has chosen the SpectraMax(R) M5e. Dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. The absorbance of the wells containing only the primary antibodies but no antigen was used as the background value. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. 91. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. G-protein coupled receptors signal through two major pathways, regulation of cAMP and increase in intracellular calcium levels mediated by IP3. With optimized reagents, option to operate the. 1 19. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. HEK293 cells in growth medium (MEM/10% FBS/1% penicillin/streptomycin) were seeded at 50,000 cells per well, 50 μL per well, in a 96-well poly-L-lysine-coated microplate. FlexStation II 384. No disponible por el momento. 0 nm increments Bandwidth (EX, EM) 9 nm, 15 nm Optimized Precision ≤ 3. 1. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Pick the best speed, sensitivity, and price for your needs. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. The SpectraMax® M5e Microplate Reader's unique mechanics allows efficient adaptation to the zero gravity in space and offers the most advanced features in one unit, making it the ideal candidate for handling research applications in a rigorous environment. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Explore PDF for more details. The resulting purple crystals were dissolved in solubilization buffer and analyzed by spectrophotometry at 490 nm, using a reference of 650 nm in a microplate reader (SpectraMax M5e, MD, USA. D 47 7. , contact Molecular. Cet appareil ISM est confomre à la norme NMB-001 du Canada. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. 1. If not, our scientific teamwill help you choose the instrument that best suits your needs and budget. 2 x 10-14. Purchase of Additional 1 Year Service Plan includes: MRC Essential Service Plan, including parts, labor, and shipping/handling costs for emergency repairs. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Microplate Readers include the M3, M4,. SpectraMax M 系列多功能微孔板读板机包括 M2/M2e、M3、M4、M5/M5e 等几款多功能酶标仪。. SpectraMax M5e: 118. The donor and acceptor can be used to label a variety of biomolecules, for. You must scan the fluorochrome of interest in the SpectraMax M5 or M5e reader to determine the optimal excitation and emission wavelengths for your application. 306 plate air cal fail,low light. The Quant-iT RiboGreen RNA Assay Kit, when run on a SpectraMax microplate reader with fluorescence detection mode and SoftMax Pro Software, is a rapid, sensitive detection method for RNA. After washing, the chromogenic substrate was added, and the OD was read using a SpectraMax M5e microplate spectrophotometer (Molecular Devices) at 450 nm. Detection modes include: • Endpoint, kinetic, spectral and well-scanning • Comprehensive data analysis • Robotics compatibility • Fluorescence Intensity (FI) • Time-Resolved Fluorescence (TRF) The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. 8. This document is provided to customers who have purchased Molecular Devices, Inc. SpectraMax M5e Reader only Certified to Cisbio Bioassays HTRF technology performance specifications Fluorescence Polarization Performance (M5/M5e only) Wavelength range 300–750 nm Wavelength selection Monochromators, tunable in 1. HTRF is a versatile technology developed by Cisbio Bioassays for detecting. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The optical systemsThe Analyst®, FlexStation® and SpectraMax® M5/M5e from Molecular Devices have received the LanthaScreen® Certified designation from Invitrogen™ which ensures that these readers are validated to strict standards in instrument seThe SpectraMax M5e reader offers the additional benefit of being certified . The plate was agitated briefly, incubated 15 minutes at room temperature in the dark and read on M5e. #6016736) ATP standards ranging from 1 µM to 1 pM were prepared as. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. WARNING! The instrument weighs approximately 36 pounds. Ex wavelength (nm/bandwidth) 485/20. Optimal instrument settings are the same for all three readers. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 6 x 10-15. 5 mP standard deviation at 1 nMF1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. The self-funded NanoRacks Plate Reader-2 features a wide range of high-performance multi. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. 00. 8 x 10-15. No special upgrade is required for HTRF ® readout, as it is a monochromator-based instrument. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Ends from. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader with One Year Warranty. Open the SoftwarePro® Data Acquisition & Analysis software,. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. Labsystems Fluoroskan Ascent FL Microplate Fluorometer. 01) + 0. Assay used: ATPlite 1step Luminescence Assay System (PerkinElmer cat. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politicsSpectraMax® M5e Multi-Mode Microplate Reader (Molecular Devices) Assay setup and detection. Enzyme titration and time course, cofactor and substrate Km,andIC50 for known inhibitors are demonstrated. View full details Original price $5,513. 5 mP standard deviation at 1 nM SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. This Molecular Devices SpectraMax M5e Multi-Mode Microplate Reader is in good working order. This document is provided to customers who have purchased Molecular Devices, Inc. Cytation™ 5 combines automated digital microscopy and conventional microplate detection in a configurable, upgradable platform. Devices SpectraMax M5e (Ex/Em 380/650nm) • SoftMaxPro software allows easy data analysis Real-time data analysis SoftMaxPro software allows easy viewing of oxygen consumption profiles in all wells (Fig.